Mouse Anti-PSCA Recombinant Antibody (clone mAb 8D11)
CAT#: HPAB-0287CQ
This product is a recombinant mouse antibody that can bind to human PSCA with high affinity.
Published Data
Gene Expression
Tested Data
FC
Figure 3 A graph comparing the amount of Surface Versus internalized anti-PSCA monoclonal antibody for each of the antibodies as measured within 1 hour, by the intensity of the Cy3 fluorescence.
FuncS
Figure 4 Shows the growth of PC-3.gDPSCA tumors in mice treated with 3 of the anti-PSCA antibodies (6F8, 8D11, and 5F2) as compared to the negative control, anti-ragweed antibody. The antibodies were administered by intraperito neal injection starting one day prior to inoculation with PSCA-transfected PC-3 cells, followed by biweekly injec tions following cell inoculation. “RX” under the arrows indicates the time point (day) of injection of the antibodies into the mice.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30783/1550_B1_8_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in subset of renal tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/ihc_selected.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_6_1.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56418/132825_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CAPAN-2, HeLa, OE19, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167653-PSCA/subcellular
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SDS-PAGE
Figure 1 Mouse Anti-PSCA Recombinant Antibody (HPAB-0287CQ) in SDS-PAGE
Lane 1: Non-reduced mAb
Lane 2: Reduced mAb
SEC-HPLC
Figure 2 Mouse Anti-PSCA Recombinant Antibody (HPAB-0287CQ) in HPLC
The purity of HPAB-0287CQ was greater than 95% as determined by SEC-HPLC.
ELISA
Figure 3 Mouse Anti-PSCA Recombinant Antibody (HPAB-0287CQ) in ELISA
ELISA analysis of HPAB-0287CQ was performed by coating with Human PSCA Protein, His Tag.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 4 Mouse Anti-PSCA Recombinant Antibody (HPAB-0287CQ) in WB
Western blot analysis of HPAB-0287CQ was performed by loading Human PSCA Protein, His Tag.
HPAB-0287CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane1: Reduced antigen (0.1 μg)
Lane2: Reduced antigen (0.3 μg)
Lane2: Reduced antigen (0.6 μg)
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Specifications
- Immunogen
- Human PSCA expressed in E.coli
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human PSCA
- Species Reactivity
- Human
- Clone
- mAb 8D11
- Applications
- FC, IHC, Cyt, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry, Cytotoxicity and Functional Assay. Please see the Published Data for further information.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Customer Review
There are currently no Customer reviews or questions for HPAB-0287CQ. Click the button above to contact us or submit your feedback about this product.
Reliable in Research
I found the Mouse Anti-PSCA Recombinant Antibody to be incredibly reliable for my flow cytometry experiments. Its specificity enabled clear differentiation between PSCA expressing cells and controls, which is critical for my studies. The precision and reproducibility it offers have saved both time and resources.
A Must for Cancer Studies
This antibody has become indispensable in our cancer research lab. Its high specificity for PSCA ensures we get accurate flow cytometry results every time. I would recommend it to any researcher focusing on prostate and bladder cancer.
Excellent Binding Affinity
The binding affinity of this antibody to PSCA is impressive. It strengthens our confidence in the reproducibility and accuracy of high-throughput assays, becoming an essential tool in our research arsenal.
Highly Recommend for Bladder Cancer
For researchers investigating bladder cancer pathways, this antibody is a game-changer. Its reliability in detecting PSCA made significant contributions to our findings and understanding of disease mechanisms.
A Researcher's Best Friend
Having used various antibodies in the past, this recombinant product stands out for its performance in targeting PSCA without cross-reactivity issues, proving to be an excellent choice for our laboratory.
Q&As
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What applications can the Mouse Anti-PSCA Recombinant Antibody be used for?
A: This antibody is mainly used in flow cytometry, immunohistochemistry, and western blotting. Its high specificity for PSCA makes it ideal for analyzing protein expression in multiple research scenarios.
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Which diseases are related to PSCA, the target of this antibody?
A: PSCA is often linked to prostate cancer and bladder cancer. This makes the antibody valuable in research focused on these cancers and understanding their molecular pathways.
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How should the antibody be stored to maintain stability?
A: The antibody should be stored at -20°C to -80°C, and repeated freeze-thaw cycles should be avoided to maintain its integrity and effectiveness.
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How does the antibody help in cancer research?
A: By targeting PSCA, it aids in elucidating pathways involved in tumor progression and metastasis, thereby contributing to the understanding of cancer biology.
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Is there technical support available for troubleshooting?
A: Yes, comprehensive support is available for users to address any technical challenges encountered while using the antibody in their research.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0287CQ, RRID: AB_3111382)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| TAB-256CT | Anti-Human PSCA Recombinant Antibody (6B8) | IHC, FC | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-262CT | Humanized Anti-PSCA Recombinant Antibody (TAB-262CT) | FuncS | Humanized antibody |
| TAB-262CT-S(P) | Humanized Anti-PSCA Recombinant Antibody; scFv Fragment (TAB-262CT-S(P)) | ELISA, FC | Humanized scFv |
| TAB-262CT-F(E) | Humanized Anti-PSCA Recombinant Antibody; Fab Fragment (TAB-262CT-F(E)) | ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0074-CN-S(P) | Mouse Anti-PSCA Recombinant Antibody; scFv Fragment (HPAB-0074-CN-S(P)) | FC | Mouse scFv |
| HPAB-0287CQ-S(P) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 8D11); scFv Fragment | FC, IHC | Mouse scFv |
| HPAB-0288CQ-S(P) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 6F8); scFv Fragment | FC, IHC | Mouse scFv |
| HPAB-1309WJ-S(P) | Mouse Anti-PSCA Recombinant Antibody (clone 6B8); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-1900-FY-F(E) | Human Anti-PSCA Recombinant Antibody; Fab Fragment (HPAB-1900-FY-F(E)) | FC, Inhib, WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0074-CN-F(E) | Mouse Anti-PSCA Recombinant Antibody; Fab Fragment (HPAB-0074-CN-F(E)) | FC | Mouse Fab |
| HPAB-0286CQ-F(E) | Human Anti-PSCA Recombinant Antibody (clone mAb 5F2); Fab Fragment | FC, IHC | Chimeric (mouse/human) Fab |
| HPAB-0287CQ-F(E) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 8D11); Fab Fragment | FC, IHC | Mouse Fab |
| HPAB-0288CQ-F(E) | Mouse Anti-PSCA Recombinant Antibody (clone mAb 6F8); Fab Fragment | FC, IHC | Mouse Fab |
| HPAB-1309WJ-F(E) | Mouse Anti-PSCA Recombinant Antibody (clone 6B8); Fab Fragment | ELISA, WB | Mouse Fab |
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| VS-0325-FY152 | Mouse Anti-PSCA (clone 6B8) scFv-Fc Chimera | ELISA, Cyt, FC | Mouse IgG1, scFv-Fc |
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| VS-0425-YC192 | Recombinant Anti-PSCA Vesicular Antibody, EV Displayed (VS-0425-YC192) | ELISA, FC, Cell-uptake |
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